Is it professional or ethical to recommend a reviewer for your manuscript as suggested by some journals?

Should authors have a say on who reviews their manuscript taking into account a blind peer review policy?

05 October 2020 3,521 9 View

Is there heteroscedasticity in my data, and if so how can I reduce it?

I am trying to perform a linear regression analysis but I fear that my data has heteroscedasticity, thus violating the assumption of homoscedasticity which is required for linear regression. I...

14 June 2020 199 3 View

Are two values statistically significantly different if their 95% Confidence Intervals do not overlap?

I estimated the percent tree cover for two fields and obtained as an example the following values ( 95 % CI): 13.67% (11.93-15.41%) and 9.27% (7.8-10.74%) . As the 95% CI do not overlap, can I...

09 May 2020 2,359 23 View

Is Linear mixed model appropriate for assessing if there is a difference in tree density between land use types. I have a nested data?

Below is a hypothetical example of how my data looks like in excel. My actual data has 16 sites and each site has about 10 plots, so its a huge dataset). Plots are nested within Sites. I would...

12 April 2020 9,774 6 View

Advice writing an unbiased qualitative research question?

Hi, I was wondering whether kindly souls out there might be able to help me with some advice regarding the formulation of a RQ for my qualitative study please? I'm trying to refine an early...

25 March 2020 6,512 5 View

How applicable are the following models in current times?

Transformative model, Engagement model, University learning model, Responsive university model and Quality management in education model. I would really appreciate to link me to the latest...

06 July 2019 3,084 3 View

How is Reynolds number calculated?

I want to know how to determine the dimensionless numbers such as Reynolds and Nusselt numbers for my combustion modeling. Its a CH4/O2 mixture, diameter of tube is 6mm, length of 60mm. Also,...

02 July 2019 6,901 11 View

Recording very high temperatures in my model of micro combustion in porous inert medium, any helps pls?

Im working on porous media micro combustion in a cylindrical channel. i have been recording very high temperature (2000K-4800K) in the combustor and on the outer wall as well which is made of...

22 April 2019 3,712 3 View

Contamination or Brownian movement?

I noticed an odd spot within one of my polyacrylamide hydrogels. It looked like gel was missing from this spot and was down to the plastic. I zoomed in and noticed this and recorded it. What do...

10 February 2019 7,271 4 View

What is a Good Gene to Identify 4T1 Cells With Q-RT-PCR?

Hi Everyone, I am planning a study to test the effectiveness of a cancer therapy on reducing tumor volume in a 4T1 mouse model. In addition, I would like to see the effect of my treatment on the...

09 December 2018 3,257 3 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View