Presence of protein in a cell line that is used as a negative control for that specific protein?

I have quite an absurd issue. for the information: 1) I have a cell line (K), that i have seen in literature being used as a negative control for my protein (P). However, for me the cell line...

30 May 2019 3,213 3 View

Different genotyping result for the same sample under same conditions?

27 February 2019 331 13 View

Total RNA stability at room temperature (+25 C)?

Hello everyone, I'm facing a problem regarding my pure extracted RNA samples, I extracted them from tissues of rats' livers and kidneys immersed in RNAlater, using the promega SV isolation...

04 November 2017 3,737 5 View

Quality costs in different phases of slip casting?

05 October 2016 5,317 3 View

Comparing different qPCR amplicons, how to account for different length of amplicons when using SyberGreen?

Dear all, I performed several qPCR, which also worked fine, however, now during the evaluation I encountered an issue. My problem is that due to the restriction when choosing primers, I ended up...

05 August 2014 3,406 2 View

For ELISA, which capture/coating AB concentration should I use?

I am developping an ELISA, which seems to work, although I am puzzled by the titration of the capture AB. The Assay: All samples were in dublicates, volume used 50ul, except 150 for blocking,...

08 April 2014 6,566 3 View

Is there any evidence that links bacteria with oral cancer?

Certain microbes have been linked with oral cancer like HPV and syphilis. Recent research suggests that anaerobic bacteria may be linked with colon cancer.

26 August 2013 698 24 View

I focus for a training course

I'm a PhD student and I'm working on "Invasive Alien Plants in Tunisia: invasiveness and management strategies." I hope to have the opportunity to benefit from a training period in a laboratory...

17 December 2012 8,704 2 View

Can we modify the pre designed Deep networks like googlenet and alexnet to use them for signal classification?

Signal classification using Deep Learning

01 January 1970 9,370 4 View

What affects will the internet of things (IoT) have on infrastructure and smart cities region?

Is the competencies of the clever grid, smart homes, and its combined with IoT components in other public utilities, including roadways, sewage, and water transport can make a contribution to...

01 January 1970 7,751 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View