I noticed it already some time ago, that in accordance to suppliers MSDS some enzymes have actually higher activity in other-than-supplied buffer. Why do they not provide the best buffer?
If you go to http://www.clontech.com/takara/US/Products/Molecular_Biology/Restriction_Enzymes/Restriction_Enzymes_g_h/HindIII and click on Documents and load the data sheet, there is a table of relative activities. HindIII should have 100% activity in M buffer (supplied), but 200% in K buffer. So why not use K buffer instead of M?
Guanfan Mao
The suboptimal buffer which supplied with will greatly improve ease of endonuclease use, especially when carrying out in double-digested system!
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Estanis Navarro
Yes, it is to facilitate double digestions with non-compatible enzymes.
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