What research has been done on learning non-Markovian reward functions?

Recently, some work has been done planning and learning in Non-Markovian Decision Processes, that is, decision-making with temporally extended rewards. In these settings, a particular reward is...

06 April 2019 4,890 2 View

Is this contamination in cell culture?

In my human cell line, I've observed masses that look different from the cells. When seen with the naked eye, they appear to be white spots. When seen under a scope, they appear to be dense...

14 August 2018 8,480 4 View

Estimating sample size: can secondary outcome data be used where primary outcome data does not exist?

I'm estimating sample size for a future study using the primary outcome (mean and SD) from a current study. The future study will use a randomized control design with two independent 'arms' (or...

17 January 2018 4,037 1 View

Can anyone spot any possible problems with this approach to diagnosing colorectal caner?

Take a sample from the suspected area, polyp, ulcer etc. Extract RNA from these cells. Convert to cDNA and do real time PCR to determine whether the oncogene VAV3 is over expressed? (VAV3 is...

25 October 2016 9,528 3 View

What is the definition of the POMDP state value function when the reward function is defined as R:S x A x S->Reals?

When the reward function is defined as R(a,s), the value function is defined as max_{a in A} (rho(a,b) + ...), where A is the set of actions, b is the current belief state and rho(a,b) is the...

05 May 2016 9,561 3 View

Why am I getting a low ICC even though agreement is objectively high?

31 January 2016 4,730 3 View

Can anyone recommend free software which is easy to use, for computing Minimum Cross-Entropy?

I'm working with discrete distributions, and i have only one constraint (over the atomic events) on the posterior distribution. In particular, i have a prior distribution P over four atomic events...

25 January 2016 6,708 2 View

How can I derive the order of a LTI system?

16 January 2016 4,836 3 View

Any tips for transfecting neurons with siRNA in primary culture?

We would like to transfect post-mitotic neurons cultured from early human fetal neocortex with siRNA. As a trial experiment,we have tried Dharmafect 1 to introduce siRNA against GADPH and found 5%...

16 March 2015 3,512 10 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View