My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.
Chi-Chi Chou
Have you tried using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 1:1? I suggest keeping the gel pieces in ratio of 1:1 (ABC buffer : ACN) for more than 2 days to try. If it still doesn't work then you have better run your gel again. This time just stain it for a short time as long as you can see the bands then do in-gel digestion and destaine as soon as possible.
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