Hi,
I have been growing BV-2 microglia in RPMI 1640 supplemented with L-glutamine, 10% FBS and 1% penstrep and 1% sodium pyruvate. However, it has not been growing very well recently and as the cell either die or grow slowly. I tried using fresh products but it has not helped.
I would appreciate any help you would be able to provide.
Also, I thought the BV-2 cells was 24 hours and just wanted to make sure this is accurate.
Try DMEM High Glucose medium with FBS. If lack of growth still occur, check mycoplasma contamination.
BV-2 should growth fast similar like HEK293.
Hello Abdullah Iqbal
Use DMEM instead of RPMI-1640.
DMEM supplemented with 10% FBS, 1.5 g/L NaHCO3, 4.5 g/L Glucose, 4 mM L-Glutamine, and 1.0 mM Sodium pyruvate. Media may be renewed 2 to 3 times per week.
The BV-2 microglial cells are fast growing cells, with an average BV-2 doubling time of 34.5 hours.
As rightly mentioned by Leonardo Tejo Gunawan, it is advised to recheck the cells in antibiotic-free medium after at least four to six subculture passages for mycoplasma contamination. Mycoplasma contamination is difficult to detect and therefore its presence can go undiscovered for months. Routine testing ensures that you are working with uncontaminated cultures.
Best.
Thank you and do by any chance be able to reference any papers using this cocktail for growing BV-2 microglia.
Leonardo Tejo Gunawan and Malcolm Nobre
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