How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

How much of an effect does switching to a Whole foods plant based diet affect study and focus among college students?

I'm looking to write a proposal paper about the effect a whole foods plant based diet has on improving focus in studying and recall in testing among college students.

20 January 2021 323 2 View

New/Creative Methods in Qualitative Research?

I will be conducting a qualitative research into well-being and help-seeking behaviour in elite coaching and I'm trying to think of new/creative ways how to do this rather than just conducting...

05 January 2021 5,158 4 View

How to develop mathematical model for Eye-Gaze tracking system?

Gaze tracking technologies are used in a wide variety of applications. Most gaze tracking systems require a personal calibration process where the user is instructed to gaze at certain points...

03 January 2021 9,675 5 View

Which is better to focus on teaching or research for a good academic life and Why?

Which is better to focus on teaching or research for For a good academic life and Why?

26 December 2020 5,737 9 View

What makes a strong research question?

A good research question is essential to guide your research paper, project, or thesis. It pinpoints exactly what you want to find out and gives your work a clear focus and purpose.

25 December 2020 6,145 4 View

Should you include the charge when writing out ions or ionic compounds?

I'm writing a chapter about the nitrogen cycle and discuss the different forms of reactive nitrogen such as NO3 and NH4. I also have some soil places where I discuss soil cations and others where...

22 December 2020 3,753 3 View