I have the FTIR absorption spectrum of cultured dance cells. I collect the cells and following washing the cells with normal saline, dry them on ZnSe disks (using vaccum conditions). Normal saline spectrum is taken as background. However, sometimes, all the peaks of the absorption are reversed. Can anybody suggest any dissolution for this observation?
The relationship between Absorbance and transmittance in the following: A =-logT wher A is absorbance ans T the transmittance. The log is the base 10 logarithme
The FT-IR spectra of absorbance and transmittance should be different. the differences shows on Y axis unit. For transmittance in Y axis shows in %T and the peak open to above X axis. absorbance peak shows vice verse.
If transmitance is expressed as %, you must use 2-log T(%). 2 = log 100. Remember: generally, absorbance varies between 0 to 1. If transmitance is 50%, A = 2-log(50) = 2-1,70 = 0,3
See any Analytical Chemistry book.