hi, after performing bronchoalveolar lavage in mice with PBS, I use trypan and MGG coloration to see and count the inflammatory cells in my samples, it seems like the cells are lysed, i don't know why !!!
I have a protein that is 117 amino acids long, with a 21 amino acid transmembrane domain at the C-terminus. I've cloned this protein into the pET28 expression vector in BL21, but am not seeing any...
20 May 2024 8,161 2 View
My research was published in the Italian magazine Scopus Q2 in 2021. The attachments are my research and the research of Indian researchers in 2023.
01 April 2024 5,153 3 View
I am doing research, and one of my research variables is audit risk assessment. I want to do applied studies instead of questionnaires.
18 March 2024 9,997 3 View
isolated from water and sediments samples.
05 February 2024 5,918 0 View
What is expected of future generations towards society and the nation ? What are the trends that generations aim to achieve ? for example a peaceful generation .. believes in values.. a...
02 January 2024 7,324 2 View
there is a problem in my code and i dont know what is it ? if there is available code can you help me with it
17 December 2023 7,151 0 View
Explanation of Time Series Research Design
19 November 2023 5,721 2 View
Among the tools of scientific research, quantitative and statistics..
12 September 2023 2,367 4 View
I am amplifying gene (1488 bp) from RNA genome using specific primers and run in reverse transcription PCR(RT-PCR). The result, I am getting 2 bands my target band (1488bp) and extra band with...
01 September 2023 4,965 7 View
Hi, I has a question regarding infection models- If you are inducing an infection model (bacterial) and then snap freezing the infected tissues in liquid nitrogen afterwards, does the pathogen...
18 July 2023 4,672 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View