hi, after performing bronchoalveolar lavage in mice with PBS, I use trypan and MGG coloration to see and count the inflammatory cells in my samples, it seems like the cells are lysed, i don't know why !!!
What do you know about biotechnology art?
02 March 2021 1,653 3 View
in the literature, in order to calculate this parameter , they use slide images with 20 microscopic magnification , but in our lab , we don't have this kind of microscope , can I use 10...
11 December 2020 9,559 3 View
i wanna administer cigarette smoke extract to induce emphysema in mice, i am asking for the maximum volume of intranasal administration ? can i use ether or chlorofom to anesthetize mice?
10 December 2020 6,319 4 View
In a scholar profile, in google scholar, when clicking on a link of an article for example, why some articles have a description and link to the page of the journal that publishes that article...
16 November 2020 5,216 4 View
Hello everyone, Hope you are very well. I just wonder if there is a word or LaTex Elsevier template for these two...
06 November 2020 4,504 3 View
Hybride intelligent system is good techniques used for solving diffrent optomization problems. Like classification, recognition ....etc Deep learning can be used with and hybrid with other machine...
08 October 2020 4,943 3 View
As hydrogel was prepared in acidic medium, released acid from hydrogel kills cells in in-vitro test. I am wondering to compromise such issue.
12 August 2020 9,319 3 View
Dear all, I am currently performing some western blots in a big wells gel (40ul capacity) and i am filling 40ul in each well. My problem is that two of the last wells supposed to be empty...
10 August 2020 4,613 4 View
mention the name of parasitic worm that causes river blindness?
04 July 2020 6,066 8 View
I have daily closing values of multiple stock indices and i have lists of public holidays for the whole period. I want to inspect the existence of a holiday effect. My regression equation is as...
23 May 2020 5,456 12 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View