I have made silver Nanoparticles using neem leaf extract. I have adjusted the ratio as 3 ml neem leaf extract and 7 ml 1mM AgNO3.

Please tell why the peak is coming at 380 nm (approx.) instead of 420 nm.

Also, please tell when I repeat the same experiment I got the reading to be exact 420 nm but afterwards it didn't come. It's repeatedly coming at 380 nm.

Kindly help.

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