What basic protocol can you recommend for making liposomes with a highly water insoluble drug?

I am new to liposome making. My drug (carbamazepine, mol wt. 236) is highly insoluble in the water. I tried to load it in bilayer using a basic protocol. After lipids(DMPC, DMPG, Cholesterol)...

23 November 2020 4,886 3 View

Does anyone has experience with Dextran-FITC uptake by cancer cells?

Please share the protocol for antigen uptake assay by cancer cells. I want to use Dextran-FITC to track the endocytosis by cancer cells. If anyone has the protocol or any suggestions kindly share.

25 October 2020 469 3 View

How informal learning can impact the learning culture of the organization?

Need to know specific parameters which impact the learning culture variables.

23 September 2020 233 4 View

Regression analysis for likert scale dependent variable with ordinal dependent variables , how ?

my survey responses are on likert scale whereas independent variables are ordinal. How to do further regression analysis in SPSS.

08 January 2020 6,781 4 View

The fluroscence given in luminex xmap multiplex technology remain equals to background value even after hybridization. What can be reason?

The probe is correctly binding but there is no rise in fluorescence.

08 October 2019 5,035 20 View

The right statistical test for microbiological data from food production environment?

I am analyzing the safety aspects of two food production sites producing similar products. I wish to compare these geographically distinct sites across seasons as well as processing steps. I have...

15 July 2019 550 3 View

Can anyone please suggest me code of LEACH algorithm in python.?

Can anyone please suggest me code of LEACH algorithm in python.?

02 July 2019 5,023 1 View

Nuclear fractionation protocol for ChIP-sequencing and Co-IP mass spectrometry?

The protein I am working on is a transcription factor and is abundant in nucleus. I want to do subcellular fractionation of the adherent tumor cells followed by ChIP sequencing in order to...

02 December 2018 5,978 1 View

Please suggest me the method to determine the thickness of coating on whole fruits and vegetables?

How can be the thickness of coating on fresh whole fruits and vegetables be determined?

04 November 2018 4,296 4 View

How do I understand what's oxidizing and reducing from cyclic voltammetry of my polymeric system?

Hello everyone, I am conducting cyclic voltammetry experiments on my polymeric composite system Polyimide/polysiloxane/graphene using a three electrode setup and 1M KOH electrolyte. I am obtaining...

26 September 2018 2,947 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View