please tell us about your research on infection of the cellular spheroid with a virus. How did you determine the effectiveness of infection and measure the titer of the virus? Can you share your experience and protocol? I will be very grateful.
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
I have a client who was married to a narcissist (she diagnosed him). Though they are divorced now, she has thought a multitude of times that he ex-husband has hacked into her cell phone and...
04 June 2024 3,166 3 View
Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...
13 May 2024 2,828 0 View
Hi, I have done mtt test and realized that my reagent ic50 is 50nM. Do I have to do ally cellular assay by treating with this concentration? Do we do the mtt test for this purpose? Of course I...
10 May 2024 1,314 2 View
How can one frame a set of questions to know if a particular group of persons have access to computers and mobile devices.
01 May 2024 5,331 0 View
I am writing a case report on a rapidly growing symptomatic brain tumor. The patient had an initial brain MRI which was unremarkable, and no brain tumor identified. after 47 days the patient was...
26 April 2024 6,861 0 View
I am testing a cpd inhibiting a kinase on a cellular assay, one cpd showed inhibition curve like this: don't know how to explain this, anyone can help?
20 April 2024 2,851 0 View
I have questions regarding the safe use of cell phones in animal research facilities. While I acknowledge the hygienic risk, we often spend a lot of time inside our barrier SPF facility and we...
14 April 2024 3,486 0 View