Who is the most cost effective supplier for shRNA vectors (UK & EU)?)

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What are the best cost-effective apoptosis assays?

My PhD student is working on phytochemical-induced cell death in cancer cell lines and we need to establish if the cell death is apoptosis or necrosis etc. As our budget is dwindling, does anyone...

08 September 2019 2,953 4 View

What is the best plasticware for keeping differentiated C2C12 cells stuck down?

Our cells differentiate really quickly in 2% horse serum + ITS and after 3 days they lift up as a plaque of cells at the edges. Will CellBind by Corning keep them stuck down?

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What is the most cost-effective media for culturing primary HUVECs?

I am going to perform a small study measuring responses to adipokines and have only a small funding pot allocated.

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Amit Sharma

I prefer Dharmacon (http://www.thermoscientificbio.com/dharmacon/)

James Brown

I have heard good reports but I think they are more expensive, from Origene you get 4 different shRNA vectors plus controls for £500.

Thomas Oguin

I've had great results using the Origene Trilencer RNAi system. With two of the three constructs giving me a nearly complete knockdown at 50 nM.

Thanks Thomas :)