Who is the most cost effective supplier for shRNA vectors (UK & EU)?)

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What are the best cost-effective apoptosis assays?

My PhD student is working on phytochemical-induced cell death in cancer cell lines and we need to establish if the cell death is apoptosis or necrosis etc. As our budget is dwindling, does anyone...

08 September 2019 3,064 4 View

What is the best plasticware for keeping differentiated C2C12 cells stuck down?

Our cells differentiate really quickly in 2% horse serum + ITS and after 3 days they lift up as a plaque of cells at the edges. Will CellBind by Corning keep them stuck down?

03 April 2014 4,595 3 View

What is the most cost-effective media for culturing primary HUVECs?

I am going to perform a small study measuring responses to adipokines and have only a small funding pot allocated.

01 February 2014 4,541 7 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Amit Sharma

I prefer Dharmacon (http://www.thermoscientificbio.com/dharmacon/)

James Brown

I have heard good reports but I think they are more expensive, from Origene you get 4 different shRNA vectors plus controls for £500.

Thomas Oguin

I've had great results using the Origene Trilencer RNAi system. With two of the three constructs giving me a nearly complete knockdown at 50 nM.

Thanks Thomas :)