CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

06 January 2021 244 4 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 4,983 16 View

Hot to test differences of mean(T test) on all variables in overlapping samples?

Good afternoon, I am working on my thesis on team work and I need to compare if teams differ when I adopt the rule 'at least 2 members of the team gave valid data' (19 teams in total), against...

14 July 2019 5,957 5 View

How do I Obtain Permission to administer the Servant Leadership Questionnaire?

I need to use the SLQ for my dissertation studies.

08 August 2018 1,864 3 View

Searching for articles on experimental power role manipulation in social psychology, can you recommend me some literature?

Hello everyone, Since I am interested in the concept of power and theories about it (soc.psy), I would like to find a specific experimental manipulation of it - and so far I am doing terrible! So,...

02 December 2017 6,806 3 View

Anti-FLAG-tag antibody for flow cytometry in mouse without major background signal issues?

Hi there, Can anybody recommend an antibody that works well to detect FLAG-tagged protein in mice with flow cytometry? I am considering to use FLAG tag for generating a transgenic mouse. HIS tag...

26 May 2016 3,404 4 View

Day care quality: Did anyone work with the MELQO - Measuring Early Learning Quality and Outcomes?

We heard that the official version of the MELQO is not yet ready to use. But we wondered whether maybe someone has a research version of it or some other experiences to share. We would like to...

27 April 2016 9,234 1 View

Antibody doesn't work for proteins of HEK lysate?

Hallo, I am doing some western blot experiments at the moment and have some problems. I transfected a construct in HEK293 cells and loaded a lysate to sds gel and blotted the proteins to...

13 April 2016 3,452 9 View

Has anyone used one of the gene trapping constructs form KOMP to generate floxed mice and actually gotten a positive LacZ staining of targeted cells?

Me and two of my colleagues from different labs at my institute used three different constructs targeting 3 different genes. All genes seem to get deleted fairly well but unfortunately no one of...

23 March 2015 8,975 1 View

Why do my infected cells detach in flatbeds and not one by one like they usually do?

Hello. I'm working with rotavirus infections of cells. Usually when I infect with RRV the cells die very fast (as soon as 24 h.p.i) and then they usually detach one by one and you can observe a...

17 March 2015 6,402 2 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Which one carries more value? A research paper in a Scopus Indexed conference proceedings or the same paper in a Journal without Scopus indexing?

I am looking for the suggestion among which one to pick from the above two categories for a research work.

27 February 2021 10,025 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Which indexation is better Scopus or Web of Science?

Both are very repetitive indexing agencies.

26 February 2021 9,455 4 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View