We tried with capillar tubes but almost had no success. The flowers were not covered before, though. Is there any daytime periodicity?
The best time for nectar collecting is around 10:00. However, if there is insect visitation to the flowers they should be covered at the previous evening. If the weather is hot and dry the nectar may be too thick to be collected. Then add 2μl of distilled water to each flower using a pipette, wait 1 min and collect the liquid. The amount of sugar per flower may be calculated from the collected liquid volume, and sugar concentration.
It is very difficult to collect nectar from individual florets of sunflower. One can only randomly collect it. However, it is possible to collect nectar if one is so much patient and has time to spare to collect.
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