How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

Can anyone please suggest where I can find a reference for ReaxFF parameters for PTFE(Teflon) suitable for use in LAMMPS?

Trying to simulate a ice-PTFE interaction and am unable to find a reference which actually lists out forcefield parameters for PTFE-PTFE interactions using ReaxFF or even other force fields like...

08 February 2021 9,373 2 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Should the Ice 1h crystalline structure be lost during equilibration process in LAMMPS?

I have created an Ice 1h structure and am equilibrating it 100K in NVT ensemble in LAMMPS for a timestep of 0.25fs in 3000 steps. The potential used is TIP4P/Ice. Although the simulation runs fine...

26 January 2021 9,603 11 View

What do you mean by 'excitation' of a plasma wave? What is the physical significance of this 'excitation'? Why is it necessary?

I have read numerous research papers on various mechanisms of excitation of surface plasma wave. But why do we need to excite a wave? Is it necessary? Can't the wave propagate without being excited?

25 January 2021 3,790 3 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

Running a Kruskal-Wallis test on a large sample with a likert scale on excel?

Hi everyone, I would like to run a Kruskal-Wallis test on a large sample with a likert scale. I am having difficulties figuring out how to process with the ''rank_avg'', and rank sum with such a...

05 December 2020 793 3 View

What is the relationship between pressure vacuum and pore water emptying?

Hello everyone, When I put a wet sample in a vacuum desiccator, the larger pores tend to empty. I would like to know if there is a relationship between vacuum pressure and the size of emptied...

25 November 2020 9,967 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Can you realize "top-down" and "bottom-up" ARE THE SAME THINGS at important junctures IN ONTOGENY (child development)?

Can you realize "top-down" and "bottom-up" ARE [ or certainly can, if not MUST, be ] THE SAME THINGS at important junctures IN ONTOGENY (child development)? This Question is NOT addressing YOU...

09 February 2021 5,289 1 View

Where can I find FDI data by sector/industry for Asian countries like India and China for the last 30 years?

Common databanks like UNCTAD, OECD, WorldBank and ASEAN Website did not have the data for the last 30 years.

09 February 2021 4,701 4 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

What is the appropriate role of memorization in foreign language teaching?

Research in FLT has always been concerned with the role of memory in learning and the relationship between memorization and learning.

04 February 2021 2,202 5 View

Participation in questionnaire for PhD Thesis, any English, Chinese or Spanish speakers?

Hello! I am looking for Spanish, English and Chinese native speakers to participate in my final survey for my PhD thesis. https://claudiomastrangelo4.wixsite.com/phdlinguist1/research This is...

01 February 2021 4,177 10 View