I am currently working on a project involving the morphologic identification of rumen flukes (Paramphistomum species) and require assistance with a reliable staining protocol for this purpose.

Specifically, I am looking for guidance on protocols that effectively highlight the structural features of the flukes, such as their tegument, suckers, and internal organs, to aid in accurate identification. If you have experience with staining techniques such as Carmine staining, Hematoxylin-Eosin, or any other methods that have proven effective in your work, I would greatly appreciate your insights and recommendations.

Additionally, if there are specific steps, reagents, or tips to optimize the process for rumen flukes, I would be grateful if you could share them. Any references to published protocols or personal experiences with staining methods would also be immensely helpful.

Thank you very much for your time and support. I look forward to any advice or suggestions you can provide.