While forming polymer gels by crosslinking PHPA and PEI at 80oC or higher temperatures, the gel surface starts turning yellow in some days, and eventually the entire gel turns yellow and gets weakened. Why is this happening?
Does it make a difference whether I conduct drug treatment in a 6-well plate, utilizing multiple wells and subsequently pooling the samples to achieve one million cells, or if I should opt for...
21 January 2024 9,405 0 View
It is published but that diksha is not me. Someone else diksha they have put
10 January 2024 1,167 1 View
Does GAPDH band on Western blot ever comes around 43kDa? Please see the attached image. Thank you
02 July 2023 2,077 1 View
I am planning to HEK293 cells for drug treatment. I would want to know till what passage I could use these cells? Thank you
15 June 2023 915 2 View
biological control of aflatoxin is very popular these days,I just want to know if nontoxigenic strains can lead to infection like aspergillosis, if yes then why we are using these strains for...
27 May 2023 8,023 2 View
I have a drainage network polyline feature the course of which is structurally controlled. To determine the dominant direction trends of the streams I need to plot a rose diagram. However, I am...
22 February 2023 7,648 7 View
I have submitted the review paper in this prestigious journal Science of the Total Environment and wanted to know about the time of review process and acceptance rate
25 July 2022 7,729 1 View
Hi everyone, I have a question regarding the snowball technique. Is there any software available for this type of sampling?
02 June 2022 5,613 3 View
Manuscript is related to computational simulations of plant-microbe interactions.
12 March 2022 5,968 3 View
I want to interpret my XRD data of silver nanoparticles that showing their crystalline nature but I am unable to find 2theta values beyond 40 degrees. Please help with your valuable feedback
21 June 2021 3,384 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View