Can I use nano spectrophotometer for optimizing reaction parameters of silver nanoparticles?

Hello everyone, I have prepared silver nanoparticles using plant extract and optimized its reaction parameters through UV-ViS spectrophotometer. Due to some reasons, UV_VIS spectrophotometer is...

04 January 2021 2,508 2 View

Any free + psychometrically robust I. Q. Test measuring Verbal, Numerical & Logical Reasoning?

Looking for a free + psychometrically robust Intelligence Test that measures the "g" factor + verbal, numerical & logical reasoning abilities to run a Concurrent Validity Study.

20 December 2020 4,301 3 View

How to calculate total protein from dry food materials with the available data of amino acids composition?

How to calculate total protein in dry fish if we have only the data of total amino acids present in dry fish. Is there any method to calculate?

14 November 2019 7,320 7 View

If any commodity have different price at different marketing channel so how to calculate and represent the data ?

Difference in price at different marketing channel Calculation of price Representation of data in excel

13 January 2019 8,010 3 View

Which buffers can be used to check hydrogel swelling at different ph?

I want to check hydrogel swelling at different ph values..Can I use PBS buffer for ph 3-10 range by adjusting 1M HCl and 1M NaOH

19 July 2018 7,884 4 View

Why is my condensation simulation diverging?

Hello, I am trying to simulate condensation in a horizontal tube on Ansys FLUENT, but every time my simulation diverges. I am still new with this software. Therefore, I took a paper as...

14 June 2017 3,127 7 View

T2 n0 colon cancer with lymphovascukar emboli any adjuvant chemotherapy?

would you recommend adjuvant chemotherapy for t1 t2 no colon cancer with poor prognostic factors eg poorly differentiated or lymphovascukar invasion any evidence for an against

14 April 2017 1,516 5 View

In phonological processing of a FMRI rhyme judgment task, what is the baseline and how can we define or get it?

17 May 2015 559 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View