We are doing enzyme kinetics with enzyme immobilized on nanostructure, we found that the enzyme activity is more efficient than its optimum experimental condition.
Yes, it may happen as immobilization on nanostructures makes enzyme more stable (highly surface area to volume ratio) and more active or efficient (possibly by exposing active sites towards substrate).
Are you asking whether it is reasonable for the activity of the immobilized enzyme to be higher than the activity of the enzyme free in solution? If so, my question is, how do you determine the amount of immobilized enzyme in the reaction?
Yes, it may happen as immobilization on nanostructures makes enzyme more stable (highly surface area to volume ratio) and more active or efficient (possibly by exposing active sites towards substrate).
The optimum condition can be affected because of mode of attachment of protein onto nanostructure. there might be increase in thermostability and optimum temperature or stability after attachment to the nanostructure.
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