Mr. Cs,

Please, consider the content of the following discussion toward methodology. The method is mass spectrometry (MS,) despite the ionization method. The methodology shown in the discussion, below, is applicable to a set of MS methods, so that it can be used to your MS experimental outcomes within the framework of different ionization approaches. It is applicable to MS/MS operation mode, as well.

[https://www.researchgate.net/post/I_have_prepared_a_Hydroethanolic_extract_I_want_to_analyze_the_alkaloids_which_method_should_I_use_LC-MS-MS_or_GC-MS]

P.S. Please, consider the meaning of abbreviations LCMS and HPLC used to your posting.

Dear Karibasappa Cs

I think LC-ESI-MS is the best to analyze lipopeptides. Please read the following paper "Identification of lipopeptides in Bacillus megaterium by two-step ultrafiltration and LC–ESI–MS/MS"

Thank you

Dear Karibasappa Cs thank you very much for your interesting rechnical question. I agree with Md. Atikul Islam in that LC-ESI-MS is is a highly suitable metod to analyze lipopeptides secreted by bacteria. In this context please have a look at the following relevant articles which might help you in your analysis:

1. ESI LC-MS and MS/MS characterization of antifungal cyclic lipopeptides produced by Bacillus subtilis XF-1

Article ESI LC-MS and MS/MS characterization of antifungal cyclic li...

This article is freely available as public full text on ResearchGate

2. LC/ESI-MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain

Article LC/ESI-MS/MS characterisation of lipopeptide biosurfactants ...

3. Characterization of Novel Lipopeptides Produced by Bacillus tequilensis P15 Using Liquid Chromatography Coupled Electron Spray Ionization Tandem Mass Spectrometry (LC–ESI–MS/MS)

Article Characterization of Novel Lipopeptides Produced by Bacillus ...

This article can also be freely downloaded as public full text.

I agree with the answers above that LC - ESI - MS / MS is suitable for your purpose. Particularly the MS2 spectra of the main peaks will allow you to determine the amino acid sequence to differentiate the iturin families (which includes iturin A and bacillomycins) from the surfactins since both groups have m/z signals in the range of 1000-1100.

Best regards

A simple GC-MS will suffice but more sophistication with improved technology with LC-ESI-MS/MS is giving better results, so why not? A lot will depends on your information of interest and your pocket, though.

It really depends on the question you are trying to answer, there have been hundreds of papers on Bacillus lipopeptides. We have done a lot of MS/MS on lipopeptides Article Iturinic Lipopeptide Diversity in the Bacillus subtilis Spec...