I grow adherent cells on polymeric materials and want to stain cells without using fluorescence.
The stain should be
1. water-based (no alcohols or organic solvents) in order to preserve the integrity of the material
2. intense and stain at least the cytoplasmic fraction of the cell
3. resistant to dehydration
4. stable for at least one week without the requirement for mounting
Many thanks!
Valeria
Please reformulated your question as follows:
- Histological stains are not water based ! Why??
read this link:
https://www.networx.com/article/water-solvent-dyes
The Difference Between Water-Based and Solvent-Based Dyes.
these dyes are used to paint and NOT in the HISTOLOGY.!!!!
Also :
Which water based histological stain stains the cytoplasm of my cells?
(Valeria dixit)
Use soluble instead based
Well, you must read this book:
B. Romeis Mikroskopische Technik
Also:
I grow adherent cells on polymeric materials and want to stain cells without using fluorescence.
(Valeria dixit)
What a kind of cells (HeLa, Fibroblastes, tumor cells...)
IMPORTANT, do you want to stain the cells in VIVO or in VITRO??
For in Vivo staining, some dyes will be transported in the cytoplasma and
will be found in lysosomes.
For in Vitro staining and after fixation, the dyes will stain cytoplasma and nucleolus
What a kind of dyes do you want to use?
- Basic
-Anionic
-Neutral
Also:
Intense and stain at least the cytoplasmic fraction of the cell
(Valeria dixit)
Please read the book :
Molecular Biology of the cell Bruce Alberts et al. 1983 Garland Publishers USA
Also:
resistant to dehydration (Valeria dixit).
Do you mean the use of toluene/ethanol after staining (in order to avoid a shrinking).
As you see your question remains OBSOLETE if you don't reformulate and
give the required infos.
JRG
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