Hi community,
I would like to track cells in vivo after their administration and was wondering if there is any vital staining that "survives" 5-10 days and is given further to daughter cells. I know there are better strategies, but this is kind of an spontaneous improvisation.
Thank you!
Hi Ruben,
you can use different approaches based on membrane staining (likewise LDL DiI) or labelling DNA (likewise BrdU), in My epxerience probably the most reliable is a BrdU puse and then after fixation performing immunofluoresce or immunohichemistry against BrdU, both wiht kit or simply buying labelling reagent and antibody against BrdU.
Good Luck
Hey Cesare,
thank you for your reply! How long is BrdU detectable in cells, once they are for example transplanted in an animal? Problem is that you can basically only detect one cell division, since the newly synthesized DNA will have no BrdU in it, right? So all other daughter cells will be not visible for you. Can LDL Dil overcome this issue?
Hi Ruben,
with BrdU or DiI thing change relativelly, BrdU labelled cells dilute staining every division and you can follow labelled cells for 2-3 division, with DiI is pretty the same!!!!!
Good lucke
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