Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Why do results of NCBI Translated Blast (tblastn) and Standard Nucleotide Blast (blastn) differ if given the same sequence ?

I have an amino acid sequence If I run it on NCBI tblastn I get the expected results. But If I take the same amino acid sequence Obtain the nucleotide sequence of it using...

28 July 2020 7,676 4 View

All-against-all pairwise global alignments?

Hi all, I have a collection of nucleotide sequences corresponding to one functional domain of a hallmark gene of viruses. So in principle I am working with relatively-homogeneous functional...

28 March 2020 8,869 3 View

How do you interpret Blastn results with 0 % query coverage and 100 % identity?

I have a blasn result for a whole genome sequence of a species against a particular gene nucleotide sequence that encodes a protein. For these results the e-value is a bit high but I have 100 %...

05 November 2019 3,504 1 View

I was only able to generate forward sequences for some of my matK, rbcL and ITS1 genes and will they still be useful for my phylogenetic analysis?

I sent some plant DNA for DNA sequencing and apparently, though sequences were sent back, some pairs don't create a consensus sequence. The sequencing company tried to re-sequence, bu to no...

26 April 2019 2,664 4 View

How to find out the non-aligned reads of a query fasta file consiting of sRNA sequences after running standalone blast against miRBase?

I am working with miRNAs in tea, and for finding out conserved and novel miRNAs, I have blasted the filtered data set of sRNA sequences against the miRBase. But the output only shows the aligned...

10 November 2018 7,704 5 View

Why no significant similarity found for a specific DNA sequence using Blastn?

Hi Everyone, I am working on finding the DNA sequence of the proteins with specific function. So far, I got around 5 proteins sequences which show a high similarity (> 96%). I use NCBI to...

21 October 2018 898 3 View

Why miRBase could predict the targeted miRNA?

In senior's previous paper,he use miRBase to predict the impact of SNP to miRNA binding.But miRBase main function is to use the name of the miRNA or use miRNA similar sequence to search the detail...

09 October 2018 315 2 View

What are acceptable thresholds when using BLASTn on fungal DNA sequences?

Hello, I'd like to know your opinions on identifying fungal DNA sequences using the BLASTn with regards to Max Score, Total Score, Query Cover, E-value, and Identity match %? What are common...

06 July 2018 9,896 1 View

Is there any tool to detect the presence of plasmids in fragmented genomes based on already assembled genomes (drafts included)?

Based on the lack of information from fragmented genomes, where there is not a correct description if the contig belongs to the bacterial chromosome or it comes from a plasmid, one option is to...

04 May 2018 1,672 1 View

Limited candidate miRNA, how do i increase my search for data mining ?

Hello all, i am trying to compare the results of blast to cd hit est 2d. the initial run gave 15 and 10 respectively but the common miRNAs between them is 6. I want to increase the miRNAs...

03 April 2018 1,826 2 View