Dear Prof. P.B. Dehankar, 

Microfiltration (MF ) and ultrafiltration (UF),  have become routine methods for protein separation/fractionation. The development of membrane chromatography, HPTFF and electrophoretic membrane contactor enable for the complete purification/separation of proteins using membrane systems.

Attached pleas find a review article which discusses the use of the above mentioned membranes for the separation of proteins.

Membrane-based techniques for the separation and purification of proteins:

An overview

Arunima Saxena, Bijay P. Tripathi, Mahendra Kumar, Vinod K. Shahi ⁎

Advances in Colloid and Interface Science 145 (2009) 1–22

Membrane processes are increasingly reported for various applications in both upstream and downstream technology, such as microfiltration, ultrafiltration, emerging processes as membrane chromatography, high performance tangential flow filtration and electrophoretic membrane contactor. Membrane-based processes

are playing critical role in the field of separation/purification of biotechnological products. Membranes became an integral part of biotechnology and improvements in membrane technology are now focused on high resolution of bioproduct. In bioseparation, applications of membrane technologies include protein production/

purification, protein–virus separation. This manuscript provides an overview of recent developments and published literature in membrane technology, focusing on special characteristics of the membranes and membrane-based processes that are now used for the production and purification of proteins

Hoping this will be helpful,

Rafik 

As Dr.Rafik mentioned you can do the protein separations using Micfrofilration and Ultrafiltration using different type of polymer membranes. thank you Dr.Rafik

Yes.

Microfiltration and Ultraflitration can be used, but UF systems are much better

Yes.

Microfiltration and Ultraflitration can be used, but UF systems are much better

Yes.

Microfiltration and Ultraflitration can be used, but UF systems are much better

Yes.

Microfiltration and Ultraflitration can be used, but UF systems are much better

Ultrafiltration (UF) is the best method to separate proteins samples. There is a variety of membranes available for Ultrafiltration separation with different Molecular weight cut off(MWCO). We can use different MWCO membranes with respective molecular weight of proteins. I have been used different MWCO polysulfone membranes with different proteins. In addition, if you give more information from  your side, it is easy for us to provide suitable suggestion. 

In my opinion, maybe you can use Polyelectrolyte Multilayer Nanofiltration membranes that can be produced by coating of Ultrafiltration membranes

In my opinion, maybe you can use Polyelectrolyte Multilayer Nanofiltration membranes that can be produced by coating of Ultrafiltration membranes

Why don you precipitate your protein with ammonium sulphate ?

Thank you to all for valuable information.

Ultrafiltration would do.