Insect Lipase Activity?

Hi everyone! My name is Nacho. I am trying to assay lipase activity from insect midgut supernatant, and I have some problems and some doubts about it. I do not have the pH-stat. Thus, the...

22 November 2020 5,595 3 View

Is Tetrabutylammonium hydroxide Mass Spectrometry compatible?

Hello forum, I would like to know if TBA.OH is volatile in mixtures of acetonitrile or methanol. I want to analyze peptides that were previously dissolved in TBA.OH-conatining solution, but dried...

05 October 2020 6,936 3 View

Any good manual for phenomenological approach to sustainability?

I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...

06 July 2020 9,618 1 View

Can RL agent's decision be explained after inference?

I would like to know if there is a method, or sets of method that can explain why a RL agent has chosen certain decision, after the agent has been trained. I'm not looking for different RL...

23 April 2020 151 3 View

Can you desalt peptide digests with high pH RPLC?

Hello! I am working with some materials that require desalting of the peptide samples prior testing. My samples contain a salt of potassium. With a standard sample, I would use R3 or C18 resins...

19 January 2020 6,772 3 View

Methods available to calculate animal density by means of camera trapping?

We have two large camera trap data sets (tropical and temperate forest), which I would like to use for estimating animal density. Which are the available methods? Strengths and weaknesses? Thanks

16 January 2019 5,879 3 View

I have to remove RNA from DNA samples. Using a RNAase A (4 mg/ml), which volumen do you recommend to use?

I have some sample that I have to re-purify and some samples to isolate DNA de novo. In both cases I have to use RNAase to remove RNA. I tried with 1.5 ul of RNAase A (4 mg/ml) but the RNA is...

13 December 2018 860 2 View

Available shapefile of marine sea temperature hotspots?

Dear researchers, someone has a shapefile of marine sea temperature hotspots worlwide desribed in Hobday & Pecl (2014). Thanks in advance for sharing. Regards Ignacio

17 October 2018 7,500 1 View

Seminal papers about digital platforms?

25 June 2018 6,246 2 View

How bad is to have frozen cells at a DMSO concentration to DMEM of 2% instead of 10%?

20 April 2018 4,639 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View