Basically I need a strain to transform it with some exogenous gene, I need a strain which should grow fast in less than a day, should have protocols to transform it, can be grown in an open system i.e municipal or agriculture waste, to convert it into biomass. the biomass will then be used for biofuel production. remember there should be high lipid content in biomass of that strain...
kindly guide me with such a strain which i can use in my project.
thanx in advance
#Microalgae #Metabolicengineering #biofules #Geneticengineering
When it comes to fast growth and resilience, go with green microalgae. Consider, Chlorella spp., Scenedesmus spp., and Selenastrum spp. I have worked with them and they are promising for simultaneous bioremediation and biomass production for high value products. The genome of Chlamydomonas has been sequenced, and there are several mutants out there, but its outdoor performance is still a challenge. Maybe you can give it a trial. Good luck💪💪💪
Hi,
I have worked with Cladophora, which is one of the common green algae found around polluted rivers. Compared to other algae, Cladophora is less explored. However, in my experience, with choice of the right medium, the growth rate of Cladophora, especially if cultivated in open tank is really high. It is a good candidate for biodiesel production, which we tried. I have read that it is good for bioremediation, but haven't tried it personally. But it is macroalgae.
Also, completely agree with Dr. Chia on Chlorella and Scenedesmus.
Hope this helps you a bit! Good luck!
My opinion, based on work over bout a decade, is that trying to choose a strain is a waste of effort. If you grow it in open tanks, wild species turn up, and by an large, it does not matter. If you want microalgae that grow fast, it is wrong to go after biodiesel. To grow fast, it needs plenty of nutrients, but in a nutrient-rich mix you get protein-rich algae. There are lipids in there, but they tend to be bonded to something else, such as with phospholipids or lipoprotein. I found that with one batch, the extracted yield was about 1% lipid. If you were prepared to carry out extensive hydrolysis the yield of fatty acid went up to over 20%.
My choice of professing method was hydrothermal liquefaction. The lipids give you diesel straight away, but there is also a rich petrol stream and a number of valuable chemicals . The work-up, unfortunately, is complicated, but I believe right now there is no process that can make fuel from microalgae economically without getting a good financial return form the remaining 80% of the algae.
Scenedesmus quadricauda, I have a recent work on that.
I also have works on Chlorella vulgaris..