What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Is there any research paper about integrating mobile application like google classroom in teaching?

This is to support or serve as a guide for my thesis regarding 21st century teaching strategies. Thankyou so much in advance!

07 December 2020 8,440 4 View

What is the common method for the analysis/identification of phytohormone-like compounds ?

Hi fellow researchers, Currently i'm doing some research in fungi-secreted phytohormone-like compounds (possible bioactive ones), and i want to know which method (for example, HPLC or GC-MS)...

06 March 2020 9,946 3 View

Essential books for philosophy of science, mind, and related?

What are the most recommended books/articles in these areas?

17 February 2020 4,250 5 View

What are the fundamentals of Locked-in Syndrome?

What are the possible etiologies, signs, symptoms, etc., of this syndrome? What is the impact on the subject awareness, state of mind, and brain function?

31 July 2019 1,801 2 View

How to calculate the volume of a pbr reactor for the production of synthesis gas?

I am designing a pbr reactor for the production of sinteis gas The data are: GHSV at stp: 5.1 * 10 ^ 5 cm3 g-1 h-1 conversion: 0.751 Flow of food: 15083 kg / h time on stream 4h Catalyst density:...

21 April 2019 8,924 1 View

What are the classic papers about edge effect in the forest?

Hello everyone! I'm looking for information about edge effect and I want to start reading the pillars about this topic, especially its influence on the vegetation structure and environmental...

01 November 2018 5,225 5 View

What are the best TLR4 & MyD88 antibodies one could use in co-immuniprecipitation (lysed brain tissue)?

We will be lysing mouse brain tissue for the co-IP of TLR4 and MyD88, followed by a western blot.

23 October 2017 7,703 2 View

How obtain HPLC grade water less expensive?

How to get HPLC grade water a little cheaper or does anyone know where I can find it cheaper?

14 September 2017 5,492 8 View

Are there data visualization tools in Big Data? What would be the methodologies or evaluation technique to determine which is the best?

I am currently doing a literary review on Visualization Tools for big data, to establish a state of the art, and later define or use a method of evaluating those tools.

07 August 2017 2,639 7 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View