Which staining method for hematoxylin and eosin is best?

Alberto Y Limón-Flores @Alberto_Limon-Flores

01 January 2018 1 8K Report

i'm interested on prepare my own solutions

From what I remember, Eosin is best made fresh as it goes moldy in solution, hematoxylin, naturally ripened preserves better in the mount

Badges
Science method

More Alberto Y Limón-Flores's questions See All

Is it possible to improve the reforming section of the Haber-Bosch process without using air separation units?

A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...

01 August 2024 7,051 5 View

My publications are linked to another person. How can I correct this?

I just joined to ResearchGate but I realized that several of my publications are linked to other "Marcos Andrade (Universidade Presbiteriana Mackenzie)". Please help me claim my work

14 May 2024 1,053 1 View

Is there any real advantage in co-electrolyzing CO2 and H2O in liquid phase to produce syngas versus using separated electrolysis of H2O and CO2?

Electrochemistry is a pathway to convert CO2 into valuable products such as CO. However, a potential side product in the cathode is H2. I wonder if it is possible to tune the composition of the...

12 May 2024 9,061 3 View

Can pulsed electric fields be used to generate heat?

Pulsed electric field is considered a "non-thermal" method for food processing that involves the application of short high-voltage pulses to create pores in cell membranes. However, since I am not...

13 March 2024 2,624 6 View

How to dissolve Potassium Clavulanate in MES buffer or water?

I read that potassium clavulanate is water soluble. I tried to dissolve it to a very low concentration but the liquid remains cloudy and a white powder is visible at the bottom of the vial

06 March 2024 8,900 0 View

Greetings!, Can anyone recommend a methodology to maintain water stress in maize, at a laboratory level?

I've seen different concepts in various articles, where they mention "maintaining 50% field capacity" or around 30% humidity. But how do I get to that information?

30 January 2024 8,168 2 View

What statistical technique could I use to analyze the cytotoxicity of a treatment in mice PBMCs?

Hello! I was working with mice primary cell cultures to evaluate in vitro cytotoxicity of a treatment, I have an n of 10. The problem is that I don't know what technique to use because when I try...

27 January 2024 6,207 1 View

What role does ongoing professional development play in enhancing the performance of preschool teachers?

Good Day! I'm Margienalyn S. Flores. A Master of Arts in Educational Management. May I ask a question regarding your study because there are some parts of it that I'm interested in, "What role...

09 January 2024 399 7 View

How does the professional preparation of preschool teacher differ between public and private school?

Hi! I'm Margienalyn S. Flores, a master of arts in educational management student. I'm interested in your study, I would like to ask question regarding your study, How does the professional...

09 January 2024 9,421 3 View

Is there any open-source software for SDS-PAGE gel analysis compatible with Apple computer?

Do you know of any of this software that is compatible with Apple computers? I tried GelAnalyzer4, PyElph, and the free online gel analyzer, but it is not working anymore. What would be the...

28 November 2023 4,236 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View