My lab partner is trying to figure how to read a KL-6 kit that comes with a round bottom plate. Our lab has a machine that only reads flat-bottom plate. Any suggestions?
Von Kassa stains for phosphates (some say calcium, but that is not correct). Please, can I get any advice on any available kit(s) that stains for phosphate? A million thanks.
02 March 2016 6,289 2 View
Our team is trying to determine the possibility of executing IHC on a slide previously used for histology or vice versa. Thank you :)
02 March 2016 1,702 9 View
I am trying to see the possiility of utilizing the endonuclease activity of bacteria as a therapeutic means for HIV. If I can get the HIV virus to fuse with a bacteria just like it does with...
05 June 2014 5,473 3 View
I am thinking of possible means for killing the HIV virus with a bacteria and subjecting the bacteria to an appropriate antibiotic.
03 April 2013 3,942 11 View
I am currently investigating methods for RNA/DNA/miRNA extractions for best qualitative micro arrays expression studies.
03 April 2013 9,221 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...
01 August 2024 8,368 2 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View