Von Kassa stains for phosphates (some say calcium, but that is not correct). Please, can I get any advice on any available kit(s) that stains for phosphate? A million thanks.
Dear Michael,
The followings are links to phosphates kit:
http://www.alstembio.com/antibodies/Alkaline-Phosphatase-Staining-Kit
http://www.biocat.com/products/PMC-AK04F-COS-BR
https://www.merckmillipore.com/INTL/en/product/Black-Gold-II-Myelin-Staining-Kit,MM_NF-AG105
Hoping this will be helpful,
Rafik
Hello Rafik,
A million thanks for this information.
Michael
Our team is trying to determine the possibility of executing IHC on a slide previously used for histology or vice versa. Thank you :)
02 March 2016 1,702 9 View
My lab partner is trying to figure how to read a KL-6 kit that comes with a round bottom plate. Our lab has a machine that only reads flat-bottom plate. Any suggestions?
06 July 2014 2,415 0 View
I am trying to see the possiility of utilizing the endonuclease activity of bacteria as a therapeutic means for HIV. If I can get the HIV virus to fuse with a bacteria just like it does with...
05 June 2014 5,473 3 View
I am thinking of possible means for killing the HIV virus with a bacteria and subjecting the bacteria to an appropriate antibiotic.
03 April 2013 3,942 11 View
I am currently investigating methods for RNA/DNA/miRNA extractions for best qualitative micro arrays expression studies.
03 April 2013 9,221 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...
01 August 2024 572 0 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View