As it is obvious lactic acid bacteria are fermentors and they shouldn't possess catalase enzyme? But from MRS agar we had isolated a lactic acid bacteria which is strictly catalase positive. How could it be?
This is very good question.Some lactic acid bacteria species can form catalase activity.This is a phenomena lied within heterofermentative lactic acid bacteria.It could be pseudocatalase activity. I found it with Lactobacillus plantarum from yoghurt.It depends on the substrate used as an energy source.It could be obtained with few glucose or if hematin is used in some cases. However in MRS broth you could find few catalase positive LAB species. Any how note the following
Whittenbury R.1984.J. General Microbiology.35,13-26.
I am also faceing the same problem but still i am getting less number of catalase negative bacteria. is the growing condition anaerobic/aerobic has any effect of catalase test result??. .is it necessary to maintain anaerobic condition???