Which seprating column is more efficient? AccQ.Tag or Pico.Tag for amino acid quantification?

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Please suggest the role of low and high molecular weight Na-alginate powder for the encapsulation of phenolic acids ?

Na-alginate powder available in two forms (low and high molecular weight) and both are available for the preparation of Ca-alginate beads. I want to know that how it will affect the encapsulation...

31 December 2018 9,142 2 View

How can higher antioxidant activity of a extract related with anti-cancer potential?

As free radicals are responsible for cell damage, then it should be safe for cancer cells as well. Anti-cancer agent means something that cause damage cancer cells. Then how could it is possible...

05 June 2018 4,648 1 View

How +/- or higher/lower zeta potential of nano-emulsion particle affects absorption rate in the gut?

What is the mechanism behind the absorption of bio-actives molecules released from nano emulsion in the gut in case of higher or lower value of zeta potential. And what is the optimum range of...

02 March 2018 1,194 1 View

What's more important? Quality of experimental work or over expressive english writing skills ?

Now a days we can see high quality of work in lower impact journals, while papers containing fewer work were published in higher well renowned journals. It eventually demoralize the scholars...

02 March 2018 4,443 13 View

How to calculate the phenolic/flavonoid content in oil/water nano emulsion?

I usually centrifuge the nano-emulsion, take out the aquaous media and add same vol. of methanol followed by vortex and ultrasonication. A clear supernatant sol. of methanol was obtained after...

01 February 2018 10,153 4 View

How to make the continuous suppy of N2 gas during the bound phenolic extraction?

It is mentioned that N2 gas is provided during the alkaline hydrolysis of plant matrix or residual part for the extraction of phenols. I made some arrangements using N2 gas balloons but there is a...

01 February 2017 2,624 1 View

What is the possible reasons behind the no zone of inhibition in 70% ethanolic extract?

A 70% ethanolic plant extract dissolved in 70% ethanol was prepared for anti-microbial activity (zone of inhibition) with 1x10 pow.7 cells per mL of klebsiella. But no zone of inhibition was...

11 December 2016 4,583 5 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

Markus Christ

Hi Vikas,

maybe this will be helpful to you:

Article Ultra-performance liquid chromatographic analysis of amino a...

Article Sensitive and Rapid Method for Amino Acid Quantitation in Ma...

Article A novel quantification method for analysis of twenty natural...

Vikas Dadwal

Thanks Markus, these articles are really helpful