How to make the continuous suppy of N2 gas during the bound phenolic extraction?

02 February 2017 1 3K Report

It is mentioned that N2 gas is provided during the alkaline hydrolysis of plant matrix or residual part for the extraction of phenols. I made some arrangements using N2 gas balloons but there is a huge loss in this assembly. Can anyone tell any substitute of gas or any modifications in the process to do so??

Catherine M G C Renard

You can also use argon, it has the advantage of being really heavier than air and thus give a bit more protection betwen when you finish purging and when the tube is actually sealed (or opened).

Badges
Science topic

More Vikas Dadwal's questions See All

Please suggest the role of low and high molecular weight Na-alginate powder for the encapsulation of phenolic acids ?

Na-alginate powder available in two forms (low and high molecular weight) and both are available for the preparation of Ca-alginate beads. I want to know that how it will affect the encapsulation...

31 December 2018 9,011 2 View

How can higher antioxidant activity of a extract related with anti-cancer potential?

As free radicals are responsible for cell damage, then it should be safe for cancer cells as well. Anti-cancer agent means something that cause damage cancer cells. Then how could it is possible...

05 June 2018 4,524 1 View

Which seprating column is more efficient? AccQ.Tag or Pico.Tag for amino acid quantification?

Kindly provide a suitable research article or link as well if possible.

03 April 2018 2,342 2 View

How +/- or higher/lower zeta potential of nano-emulsion particle affects absorption rate in the gut?

What is the mechanism behind the absorption of bio-actives molecules released from nano emulsion in the gut in case of higher or lower value of zeta potential. And what is the optimum range of...

02 March 2018 1,070 1 View

What's more important? Quality of experimental work or over expressive english writing skills ?

Now a days we can see high quality of work in lower impact journals, while papers containing fewer work were published in higher well renowned journals. It eventually demoralize the scholars...

02 March 2018 4,333 13 View

How to calculate the phenolic/flavonoid content in oil/water nano emulsion?

I usually centrifuge the nano-emulsion, take out the aquaous media and add same vol. of methanol followed by vortex and ultrasonication. A clear supernatant sol. of methanol was obtained after...

01 February 2018 10,023 4 View

What is the possible reasons behind the no zone of inhibition in 70% ethanolic extract?

A 70% ethanolic plant extract dissolved in 70% ethanol was prepared for anti-microbial activity (zone of inhibition) with 1x10 pow.7 cells per mL of klebsiella. But no zone of inhibition was...

11 December 2016 4,458 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View