It depends on the size of the proteins you want to resolve. In general, tris-glycine is used for separating protiens >30 KDa where as tris-tricine is used for

It depends on the size of the proteins you want to resolve. In general, tris-glycine is used for separating protiens >30 KDa where as tris-tricine is used for

majorly it depends on the size of the protein you want to separate/resolve by using SDS page.

I am agree with Mr. Ramachnadramouli Budida,

Under normal conditions, we can use pH8.8 Tris-Gly buffer.