I'm studying cell survival in primary dopaminergic neurons upon toxin challenge with MPP+ (in vitro Parkinson's Disease model). I want to test whether a range of compounds can reduce dopaminergic cell death in this assay.
I'm in doubt how I should handle the compound to protect myself and my colleagues sufficiently?
You must be careful not to accidentally ingest the compound powder or solution, in addition to avoiding contact with the skin because it is easily absorbed. The use of gloves, safety glasses and a mask is recommended.
Finally dispose the residuos of in an organic waste container.
Okay, thank for your answer! I need to incubate my cells with the compound at 37 deg for 48 h. I'm in doubt whether MPP+ evaporates from the well plate? do you happen to know whether it is safe as long as there is a lid on the plates?
In this case, I will not know how to give you information, I work on mice, but I would expect it to be dissolved, depending on the boiling point of the solvent, but having a cover on the culture plate, subliming there. Just take the precautions I mentioned before wearing a mask, gloves, safety glasses and lab coat.
Hello Marloes,
MPP+ is the metabolised active form of MPTP. While not as potent as the parent compound in crossing biological barriers, the usual protective measures and good lab practices (Nitril gloves, mask, glasses, a dedicated lab coat, marking off a shelf and you culture plates with signs, avoiding touching surfaces outside the flow with contaminated gloves, etc.) is well worth following. Always clean your work spaces and neutralise any liquids containing MPP+ with 1% bleach, before disposing in the biohazard liquid waste.
Ref to Article The parkinsonian toxin 1-methyl-4-phenyl-1,2,3,6-tetrahydrop...
for further info., as well as the manufacturer’s MSDS, and to be on the safe side, consult your University’s safety department for guidance on usage and disposal practices.All the best!
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