I constructed two plasmids with different reporter gene (mHERRY and sfGFP). This will be used later on to quantify amount a certain intermediate in a metabolic pathway that contributes to decrease in pH level of the medium. I am just worry about the stability of my reporter protein when the pH is low.
Hi Angelo, if you are transfecting eukaryotic cells, I would be more worried about proteasome degradation than pH changes.
I would imagine mCherry is more stable to low pH than sfGFP,. This is at least the case when comparing the mCherry fluorophore with EGFP, as the latter is more sensitive to low pH.
Thank you so much Dr. Stefansson for the insight! I am actually dealing with E.coli metabolism. Do you have any idea?
Hi Angelo, sorry, I didn't know you were working with bugs.
This is not my forte, but please avoid the proteins being over-expressed and deposited into inclusion bodies.
Inclusion bodies are a mess of denatured proteins that will probably be of no use to your research.
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