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Questions related from Angelo Banares
I am constructing a plasmid with my test genes under constitutive promoter and I will be using T7 as their terminator since it is already in the plasmid that I am using. My host strain does not...
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I am working on the promoters that bind to new to nature transcription factors. I read journals and they usually used EMSA for this kind of work. I just want to ask, if DNA affinity chromatography...
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I am measuring the activity of my promoters in response to different sugar substrates. I placed the reporter gene downstream of the promoter. FACS is not available in our laboratory, so we used...
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I constructed two plasmids with different reporter gene (mHERRY and sfGFP). This will be used later on to quantify amount a certain intermediate in a metabolic pathway that contributes to decrease...
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