Angelo Banares

4 Questions 8 Answers 0 Followers

Questions related from Angelo Banares

Do you have any idea if the RNA polymerase in E.coli can recognize T7 Terminator?

I am constructing a plasmid with my test genes under constitutive promoter and I will be using T7 as their terminator since it is already in the plasmid that I am using. My host strain does not...

06 June 2018 9,237 0 View

Which is a better method for identification of DNA Binding protein, EMSA or DNA affinity chromatography?

I am working on the promoters that bind to new to nature transcription factors. I read journals and they usually used EMSA for this kind of work. I just want to ask, if DNA affinity chromatography...

08 August 2017 329 0 View

Is it enough to use fluorescence spectrometer to measure the fluorescence in cells?

I am measuring the activity of my promoters in response to different sugar substrates. I placed the reporter gene downstream of the promoter. FACS is not available in our laboratory, so we used...

08 August 2017 4,245 4 View

Which reporter protein is more stable to low pH? mCHERRY or sfGFP?

I constructed two plasmids with different reporter gene (mHERRY and sfGFP). This will be used later on to quantify amount a certain intermediate in a metabolic pathway that contributes to decrease...

07 July 2017 8,406 5 View