As part of my research proposal I am looking for an accurate method to write down for an in-vitro test on MCF-7 cell line.
Hi Mariyam Ejaz Attique
First, the question seems incomplete. There is n number of in-vitro tests available to do that also not only with MCF-7 cells, but you can also do with any cell type.
What are you going to study, say in which area are you going to write your proposal?
I know this is cancer cell but in-vitro test in which angle: apoptosis, proliferation, ROS, mitochondria, antioxidants, gaseous mediators, cell cycle, ATP, enzymatic activity, glucose metabolism, drug resistance, angiogenesis, migration, invasion, etc.
So many in-vitro tests are available to study cancer. Now, which one you are looking for?
Hi Saurabh Mandal
Thank you very much for the response. I am specifically looking at apoptosis. The apoptic effect of a product on this cancer cell line.
hi
Then you can look for these assays,
-Apoptosis: Caspase 3/7 assay, Cytochrome c ELISA, Annexin V/PI staining, Calcein AM
--Mitochondrial status in apoptosis: JC-1 assay, TMRE staining
---ROS assay
----DNA level: Hoechst 33342, TUNEL assay, BrdU assay
Now, these tests will need of fluorescence microplate, fluorescence microscopy, and flow cytometer. Based on resource and instrument availability, you can propose any of these assays.
Regards
Saurabh
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