Which procedure is effective in Extracellular Polymeric Substances extraction from marine biofilm forming bacteria?

More Aboobucker Siddik Abul Kasim's questions See All

What is polymer/chemicals can increase the swelling index of a polymeric film?

Polymeric film such as latex rubber substrate or polymer

01 July 2024 8,343 4 View

Can Metal-Organic Frameworks (MOFs) use as a primer on metallic monoliths?

FeCrAlloy® foils are commonly used for fabricating metallic monoliths. However, their lack of specific surface area/roughness (pores) hinders catalyst adhesion during the coating process. Several...

22 May 2024 8,462 1 View

How to download Lung cancer (LUSC) gene expression data from TCGA(https://portal.gdc.cancer.gov/repository)?

How to download Lung cancer (LUSC) gene expression data which includes tumor tissue samples and corresponding control tissue samples from TCGA(https://portal.gdc.cancer.gov/repository). Any...

13 May 2024 2,481 1 View

Which method would be best for calculating runoff estimation where the area is greater than 200 km2?

If anyone want to know about this topic you can ask me.

23 March 2024 5,008 5 View

How can I Apply dirichlet and zero flux boundary condition for different times in Comsol?

In one of the boundaries, Saturation, S =1 for time (t

19 March 2023 9,715 1 View

What is the reason of turbid or milky serum? How to get rid of cloudy serum?

I have been working with a transgenic mice which receive seven days treatment with an antibiotic. After seven days I collected blood in the serum collection tube. After centrifugation I found...

04 February 2023 1,011 1 View

Is it possible to fix topological errors by writing code? Does anyone know of such a study?

I'm having trouble doing network analysis due to topological errors. I am using ArcGis software.

23 December 2022 8,250 2 View

What Positive & Negative control is required for G2A Knockdown & Overexpression?

What is the Positive & Negative control for G2A Knockdown & Overexpression?

17 February 2022 2,374 2 View

How can I apply pressure(boundary condition) which is changing wtih time between two fluid interface in Ansys?

During the wicking of liquid, it goes up due to capillary force. It is required to apply pressure at the interface of liquid and air. The position of this applied pressure changes with time.

10 November 2021 5,393 3 View

Why it is not possible to detect Si-30 and P-31 isotope simultaneously using ToF SIMS??

I am using PHI nano TOF II TRIFT SIMS from Physical Electronics, MN, USA. I have grown Phosphorus doped silicon thin film (200nm) on glass using HWCVD. I need to detect P-31 isotope along with the...

30 March 2021 1,346 4 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

Is there research on the use of polymeric materials in dams?

19 June 2024 1,135 1 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

What are the laboratory precautions taken by the user when handling cancer cells or cancer cell derived extracellular vesicles?

Please give some insight on this

06 June 2024 2,016 2 View

What method is good for doing in vitro degradation study of PCL microsphere by change in weight calculation or change in diameter (from microscope)?

I have prepared some polymeric microspheres from chitosan and PCL. However while performing in vitro degradation study, I found difficulties in both above mentioned methods. Here I am mentioning...

20 May 2024 6,114 4 View

Why polymer material exhibit three peaks in zeta potential measured using DLS?

Why does a polymeric material exhibit more than one peak in zeta potential measured using DLS? The material shows a single peak in acidic conditions but displays three peaks in basic pH. What...

17 May 2024 6,439 7 View

Will ultracentrifugation be replaced as the most widely used method for isolating extracellular vesicles?

Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.

16 May 2024 9,432 1 View

Can anyone suggest damage model for polymer materials in Abaqus?

Hi, I simulated the experiments in Abaqus. In order to see the damage propagation until failure in Abaqus for polymer materials, which damage models can be used?

25 April 2024 9,342 0 View

Why I fail to inhibit extracellular microvesicles?

I tried to inhibit extracellular microvesicles(MVs) secreted from cells, through using Rock inhibitor(Y27632), calpeptin and several siRNAs(siCDC42, siTSG101, siARF1), but failed to decrease MVs....

23 April 2024 7,369 0 View

Padmavathi Alwar Ramanujam

You can extract the cell bound EPS using 20mM EDTA followed by isopropanol precipitation. It works well and it is non destructive.

Gerry/Gerre A Quinn

Comparison of extraction methods for quantifying extracellular polymers in biofilms

Xiaoqi Zhang*, Paul L. Bishop*, Brian K. Kinkle**

http://www.sciencedirect.com/science/article/pii/S0273122399001705