How to convert counts to concentration in ToF-SIMS profile?

I have taken ToF-SIMS profile of boron and phosphorus in silicon film grown on silicon substrate. I have used diffusion furnace for boron and phosporus diffusion. Please see the sample curve I...

25 August 2020 5,541 7 View

Dear Respectable members of RG, What should countries do different to curb the further spread and complete eradication of COVID-19?

The continued spread of COVID-19 across the globe is worrying. Especially in Africa specifically Ghana the reported cases keep increasing. In Ghana, on-going voter registration exercise and...

02 July 2020 9,278 4 View

How to fabricate a uniform pinhole free thin film CdS via CBD?

What precursor materials should I use for Cadmium and Sulphur and what are the optimal deposition conditions (i.e. concentration, time, temperature and pH level) for the synthesis of Cadmium...

23 July 2019 6,451 3 View

Anyone help me to real time data analysis?

we optimized the gene expression with different condition and different concentration of the sample and we obtained the ct value in the range of above 26-30. The ct value for Housekeeping gene...

06 January 2019 6,677 2 View

How can I get conductivity from the impedance analyzer (AC). I got resistance from the machine reading. How to convert it to conductivity?

Is it possible to measure directly the conductivity from the impedance analyzer?

24 February 2018 6,306 2 View

How much important to know the OC% and CaCo3% in soil while studying about heavy metals ?

To study the heavy metal presents of a sediment in the water body. is it necessary to determine the OC% and CaCo3% in the particular sediment sample. How can we relate both.

23 November 2017 1,833 9 View

Hi everyone, i have question regarding XPS. If we have sample SiC thin films, is it ok to use adventitious carbon for charge correction?

I have read some article of SiC thin films. They used adventitious carbon for charge correction. My question is, since SiC already contain carbon, so how can we used carbon as charge...

27 August 2017 8,810 11 View

In SEM, a dielectric specimen coated with a metallic.

13 July 2017 3,824 3 View

What is the different between the words spectroscopy and spectroscope?

09 July 2017 2,348 9 View

How it can be proved that biennial onion has converted to annual?

02 July 2017 6,668 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Scanning Electron Microscopy for Extracellular Vesicles?

Can anyone provide or recommend a detailed protocol for preparing extracellular vesicles for SEM? Most protocols that I have found that list the necessary reagents and individual steps are for...

02 February 2021 4,475 3 View

How to create a scaffold by using the particle leaching process?

In the leaching process,Why does NaCl settle despite sieving? How can we understand how much solvent the system needs? And, how can enhance the mechanical strength in this process and find the...

12 January 2021 5,679 2 View

Scherrer Constante of polymerics materials in X Ray diffraction XRD?

To calculate the cristallite size L We need K the Scherrer Constante what is mean this parameter and what is its value for the polymers

30 November 2020 6,510 7 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

Can someone please share how to add a spherical wrap / correction to a visual stimulus presented to mice using python?

Hi all I use psychopy 3 to show mice a visual stimulus on an LCD screen while preforming acute extracellular recordings in vivo. I understand that one should use a spherical wrap to the stimulus...

22 November 2020 7,905 2 View

Recommend equipment of in vivo recording in mice brain?

Hello, I am trying to set up an in vivo recording system in mice brain. Which company do you recommend? Neuralynx? TDT? NeuroNexus? Which one do you guys use and why? Please let me know. Thank you!

12 November 2020 2,508 1 View