Can I extend degenerate primers using a proofreading polymerase?

Does the 3-5 exonuclease activity degrade the mismatched primers

19 December 2016 956 4 View

Can anyone help with electrochemistry with Cu64?

23 July 2014 9,754 2 View

Very High Estimate & Standard Error in AMOS?

I am currently testing a structural moderation model in AMOS using the orthogonalization procedure as advocated by Little et al. (2006). I am using the Big Five personality traits as moderators,...

18 July 2014 3,300 6 View

Friedman anova doubly repeated

Does anybody know how to perform a Friedman analysis of variance on doubly repeated ranks

12 November 2013 630 3 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Is there any FDA approved polymer or IIG listed polymer which is easily soluble in sesame or castor oil ?

I am in search of a polymer which has a solubility in oil.

25 December 2020 9,481 2 View

What is the upper and lower limit for plate count for fungi?

I am currently trying to quantify the viable count of trichoderma spp. in a compost sample. However I do not have much literature on the range for plate count of fungi. should I be following the...

19 December 2020 8,874 6 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Is there any ingredient I an add into formula to slow down gas generation from Vitamin C?

I know Vitamin C is unstable and tends to be oxidized quickly. The problem here is I made a Vitamin C beverage at pH 4-5, and it starts to generate gas in the packaging, especially at high...

13 December 2020 6,055 1 View

Alkaline hydrolysis and extraction of Vitamin D from food samples?

Hello everyone, may I ask about the purpose of adding ultrapure water after incubation for 30 minutes at 80 degree Celsius (under saponification process of vitamin D? Secondly, what is the...

02 December 2020 8,533 4 View

B12 deficiency resistant to parenteral treatment ?

hello i have a 40 year old female patient with chronic fatigue and forgetfullnes who also has hashimatos disease wich is under control. Her serum B12 level vas 164 pg/ml. After treatment with a...

02 December 2020 6,676 3 View