Hello everyone,
I performed a flow cytomery analysis for evaluation the effect of a chimeric antigen protein on proliferation of CD4/CD8/CD29 lymphocytes. Now, I wanna know which plot(s) or data should be reported as a flow cytometry analysis?
Thanks a lot in advance
It would depend on your data.
If you are comparing the effectiveness of a protocol you may plot the mean and standard deviation of the cell proliferation rates between protocols.
If this work is the validate a technique you may look to plot mean proliferation rate in the presence of the protein compared to negative control.
You would need to include more details, such as the aim of the experiment, for further answers!
It depends on the results you want to show. I assume your chimeric antigen protein induce some kind of change in % of some of the populations (CD4 or CD8 or CD29 or some doubles). A dot plot of control condition and other with chimeric antigen protein condition with the specific populations that change, with the % of positive cells draw in the figure of each its a common figure in an article, that should be the "report of the FCM".
However, data analysis of multiples samples/replicates verifying the results shoud be performed, and could be included in the text, e.g. chimeric antigen protein (CAP) induce significant decrease of CD4+ population (Control: 25%+-2; CAP: 15%+-1; P: 0.0005)
It depend on your requirement. Suppose if you want to check dose dependent on any even use histogram and you can analyze different sample by placing same paramters on all sample at the same time. You also can see changes just by overlaying two different sample histogram on each other. If you want to study two parameters at the same time (e.g. PI and Annexine V staining for apoptosis) it can be analyzed using dot plot for two dye labelled cells. Hope attached file will help you with few examples.