What is the maximum overall column length suitable for LC-MS using a 50 mm HILIC column?

Curious about your research experiences. Ideally this is for ESI-QTOF electrospray ionisation. This will differ based on type of columns and equilibration as per dimensions, flow rate and size of...

09 October 2017 5,453 6 View

Are there any literature sources on coupling of HILIC column to another separate stationary phase?

08 September 2017 8,659 6 View

What research techniques have you learned over the years allowing you to quickly finish research?

For me cite them right website for referencing got lab reports done easy with references. Learned web of science makes results so narrow and easy to find. Google scholar let me find cited articles...

08 September 2017 5,953 2 View

I want to test my HILIC column with different mobile phase %. Do i need to equilibrate 2 hrs per mp %?

the water layer would have formed using 85%. Is the same amount of water layer formed by each acetonitrile %.what I am asking is for 97% if I equilibrat for 2 hrs then can I equilibrate for 30 to...

08 September 2017 7,296 11 View

How much of a water layer is required in HILIC column to effectively bind hydrophobic compounds?

,Tried 3% water layer but equilibration time is time consuming. Analyte had a curved out looking shape such as an upside boat that continued for 2minutes to reach the baseline. steep then smooth...

08 September 2017 4,633 9 View

For my HPLC UV detection can I apply 0.1% formic acid and 10mM Ammonium acetate at pH 4 to see what effect the buffer combo can have in HILIC?

I read in Mass spec literature that buffer combinations are attempted. Are they only for gradient elution. Does this need mass spec to ionise the buffered solvent effectively. Will this cause the...

08 September 2017 617 4 View

What are some good examples of articles for moderately polar compounds separated by HILIC?

LogP

08 September 2017 720 5 View

I have an impurity from a purchased bacterial standard. What methods do you use to analyse if it is a standard besides HPLC-UV detection?

08 September 2017 7,121 2 View

What are the typical To void volume markers used for mixed mode chromatographic columns that do not retain hydrophobically/ hydrophilically?

This situation can be applied to 2D chromatography. If there is a T-piece can two To void volume markers be used to measure that volume for sample to reach the detector. Some research applies...

08 September 2017 9,947 5 View

Are there any infra red detectors for HPLC or UV+IR detector for liquid or gas or supercritical fluid chromatography?

Just wondering about their expense to make. Does liquid propulsion cause excess interference in IR?. A multifunctional HPLC that checks for impurities would be more valuable. Does the liquid...

08 September 2017 7,457 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View