Which PCR to be used for MRSA?

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Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Anyone can list the activities based on body schema which will enhance the nonverbal communication of mentally retarded children ?

02 July 2024 9,947 0 View

How to perform electropolymerization on the screen printed carbon electrode?

I want to perform electropolymerization on 3 system carbon electrode. How to cover the reference and counter electrode? I want to make polymer only on working electrode.

23 May 2024 1,681 3 View

Which books should i read to learn the basics of Magnetic Nanoparticles?

I want to learn the Magnetism of nanostructured materials in very deep. Please suggest me some books , if anyone of you have suggestions.

18 May 2024 7,415 1 View

Electro-optical Characteristics Measurement System- LCMS. Is the VT curve means graph between Driving voltage and relative transmittance?

After measuring VT curve using Electro-optical Characteristics Measurement System like LCMS, how to plot graph. What is PD voltage and relative transmitance. Is the VT curve means graph between...

15 May 2024 2,393 0 View

How to calculate yield percentage using HPLC data?

I have depolymerized byproducts and commercialized products. I doubt how to calculate yield percentage using HPLC data. Can anyone give me an answer? It will be helpful in my research career....

29 April 2024 9,448 5 View

How to fix the molarity of 2 analytes to be interacted in Gauss View using DFT ?

I am trying to optimise the molar ratio of 2 analyte (For example A and B) during a reaction. For this purpose I want to optimise there molar ratio. For example A:B = 1:2, 1:3, 1:4, etc. Which...

28 April 2024 5,775 2 View

How to perform TEM imaging of chitosan nanoparticles using cooper coated TEM grids?

Is it possible to get a proper TEM images of chitosan nanoparticles as a film of nanoparticles are formed on the cooper grid?

16 April 2024 368 3 View

How can I develop an enzyme assay for degradation of gluten( low quantity of enzyme available)?

I need to develop an enzyme assay to evaluate the degradation of gluten using proteases. Previously, I conducted tests with finished products, which was straightforward. However, I now have to...

07 April 2024 1,886 3 View

What is the most effective enzyme for breaking down gluten formed when flour is mixed with water?

I've selected protease as the optimal enzyme for eliminating gluten formed from flour. Could you please provide insights on the best enzyme for removing gluten, dosing methods, and how to identify...

24 March 2024 1,618 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View