Different parts of the plants can be used for the isolation of genomic DNA. But which part is most preferred for DNA isolation and why?
All will be depend on your aims - what genome you interested in (nuclear, cp or mt) and what plants you work - live or extinct. But generally the next tisseus are prefered to use - apical and lateral meristems and live vegetative tissues. As for parts you may use buds, kambium, leaves, flowers, seeds and so on. For some studies pollen is also good to use.
Except aims of the research in case of choise tissue are some general critical factors: 1) tissue availability 2) quantity of integer DNA of genome you interested in this tissue 3) quantity of specific secondary metabolites which can affect to DNA isolation.
Another cruical moment - the choise of most suitable technique or kit for DNA isolation for your own aims.
Leaves and needles are the ultimate ones.
In case of old leaves and needles I recommend (if they are old)(3-4 years old needles example) to use buds. (my personal exp. is really good!)
Also people use roots and rhizomes at herbaceous plants and cambium tissue of forest trees. It depends on the purpose!
Material can be fresh, dried (silica) or frozen doesn't matter.
Reason: proteins and other contaminants accumulation can result quiet bad quality of total DNA.
All will be depend on your aims - what genome you interested in (nuclear, cp or mt) and what plants you work - live or extinct. But generally the next tisseus are prefered to use - apical and lateral meristems and live vegetative tissues. As for parts you may use buds, kambium, leaves, flowers, seeds and so on. For some studies pollen is also good to use.
Except aims of the research in case of choise tissue are some general critical factors: 1) tissue availability 2) quantity of integer DNA of genome you interested in this tissue 3) quantity of specific secondary metabolites which can affect to DNA isolation.
Another cruical moment - the choise of most suitable technique or kit for DNA isolation for your own aims.
I used both leaves and young (cca 2weeks old) roots. And I would recommend leaves as I never had any problem to obtain DNA from them.
@Andrii Tarieiev: Very well answered.
@Peyman Habibi: Even when the desired gene is expressing only in roots, the genomic DNA isolation from any part will include that gene in the genome. The question is not about RNA expression, but about DNA isolation.
I have always used fresh apical material. Avoid ligneous tissue as tannins may inhibit downstream enzymatic reactions :) Good luck!
I think young fresh material like young leaves or roots from seedlings is good.
Leaves. They contain big and elongated cells with huge vacuoles, that are easy to break. Meristem cells are harder to break, as they are smaller and more compact.
young tender leaves will be best for isolating the genomic DNA because the number of cells per unit area will be more and it may contain less un wanted secondary metabolites and phenolic compounds
younger plant parts will give a higher genomic DNA amount than older ones.
I think young leaves, because this part is more quiker grinded than other part of the plant and then, you profit by experiment time, if quality of DNA don't take into account. But, leaves shape and compunds (resin etc.) is always effective for reach to DNA.
Depend on what kind of plants are you working on! For Arabidopsis or any plant that has umbelliform or corymbose inflorescence, I would suggest you to use newly grown buds. They are very good source of DNA. Otherwise, very young green leaves could be used for DNA extraction. For poaceae family plants take special care in extracting the DNA from leaves as they have silica deposits at the abaxial side of the leaves.
Good luck
the young or tender leaves of a plant is good for good quality of DNA. Morning time is best time for leaf sample collection.
As Laxmikanta said: Young leaves are often preferred for nuclear DNA (genomic DNA), but also for plastid DNA. You have a high number of cells per weight, but very little vacuole content, meaning a high ratio of DNA/water content, and low content of secondary (especially phenolic) metabolites, which may disturb extraction, and low chlorophyll content. Easiest for many plants is to grow from seeds and take young leaves from young plants only a few days old.
it is depend on your plant leaf part is choose for DNA isolation, and suitable method to select for DNA isolation is important and finally handling is important
Some parts of the plant (woody stems) are not preferred as they are more difficult to grind than softer tissues. Roots are not a common choice due to soil contamination. Tissues containing high amounts of starch (tubers, many seeds) or secondary metaboliites are generally challenging to extract from.
The part to be used depends on the purpose as well as the method to be used. Generally, young leaves may be preferred.
Young leaves are generally preferable. they have higher number of cells per unit area.
If you are going to isolate the genomic DNA of plants then i also suggest young and tender leaves reasons have already provided by other Researchers. I also recommend CTAB method for DNA isolation.
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We use cauliflower (the vegetable). It is a huge inflorescence containing large number of meristematic cells.
Young tissues (leaves, buds) in full expansion have obviously many copies of DNA molecules. The problem occurs with wooden parts (from tree trunk or branches). According to our experience, cp and mtDNA are well preserved (in many copies) in almost any wood-tissue but the genomic DNA is better extracted from sapwood than from heartwood.
Try the leaves for 3 reasons:
1 - It's easy to isolate DNA from leaves
2 -Normally they are more abundant if you compare with other tissues/organs
3 - They have more cell/area.
I agree with my partners above, If I have plenty tissues to choose, I'd choose always young leaves or meristems and soft parts of the plant. easy to powder and high number of cell per area.
Good luck
Use leaves as you need quantity since leaf forms larger surface area ,other wise use meristamatic regions such as shoot leaves (contains soft tissue portion of the plant )
Dear all, can someone explain me why there are so many answers under a question that Google\Google Scholar answers in 0.000000001 sec. ?
In my lab we also extract DNA from a leves of the plants that are nearly 3 weeks old. If you use chloroform assay the concentrations should be very high, up to 450 ng/micro liter.
It is better to use fresh and young leaves for extraction DNA because the disruption of cell walls it is easy, these leaves are not lignified (or not contained secondary metabolites) and you can obtained a high quantity of DNA.
We are using ; young fresh leaves. Because they have less phenolic compounds and young tissue has more cells per gram.
Young tender tissues are without the doubt the best, but even wooden part of plants are prone to DNA isolation - it is just the matter of the chosen method of extraction.
For this purpose young leaves are used because leaves contains such best amount.
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