I am looking for a panel of immune cell subtypes that in your opinion are fundamenatl to understand the possible immune imbalance in the intestine and mesenteric lymph node of DSS-induced colitis mice.
Which markers for which cells?
Thank you
Dear Giuseppe,
As someone who works with innate lymphocytes, my response may be a bit biased, but I'd recommend looking at numbers of inavariant Natural Killer T-cells (Type I iNKT cells): these can be identified flow cytometrically via CD1d tetramer (very reliable) or via PCR for the murine iNKT TCR = Valpha14-Jalpha18.
Tregs are also an interesting population worth looking at. "Natural" Tregs are CD25hi, CD4+ and express their signature transcription factor FoxP3. Induced Tregs do not express FoxP3, but have high expression of CD39.
Good luck!
A.
Dear Giuseppe,
I would suggest you to have a look to this paper: http://www.hindawi.com/journals/bmri/2012/718617/
I found it quite helpful when I wrote the animal protocol for the DSS-induced colitis in mice. Hope this will help you!
Cheers,
Alessia
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