Housekeeping genes were first described as those genes always expressed in the cell (Watson JD, 1987) but isn't enough, they must be constitutively expressed in all tissues in all physiological conditions taking into account cell homogeneity or heterogeneity. So, which gene has all this features?
This question has been asked already several times in RG.
As a start I would recommend to use the RG search function and search for "house-keeping gene" you will find several threads related to your question.
It depends on the tissue, animal, etc. Try to find some information previously published about standardized HKG in your conditions, and then try a set of genes in order to see wich give you the best results.
To measure the expression stability of each HKG you can use this method
http://www.ncbi.nlm.nih.gov/pubmed/?term=Vandesompele+J.%2C+De+Preter+K.%2C+Pattyn+F.%2C+Poppe+B.%2C+Van+Roy+N.%2C+De+Paeppe+A.+%26+Speleman+F.+%282002%29.
Hi Davide, normally you could use HGPRT or other genes involved in essential processes such as glycolisis. [e cmq la prossima volta se chiami ti rispondo io :-D]
Please read this thread first:
https://www.researchgate.net/post/Which_house_keeping_genes_I_can_use_for_standard_and_to_normalize_the_expression_of_genes_of_interest_in_Real_Time-qPCR_gene_expression_analysis?cp=re65_x_p2&ch=reg&loginT=sROqiERwsTRBmw-kc5NT9ZM3TWTV1YtrYnyrkbEua6Y%2C&pli=1#view=51d74da9d11b8bb42ab20a19
Asking the same questions and getting the some answers makes RG difficult to search and not an attractive platform to find useful information.
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