For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Which is the best kit to detect urea level in primary hepatocytes culture? Any published references?

I need to measure the urea level in the cell culture system as well as culture media? Any recommendations?

02 November 2020 1,493 1 View

How to calculate various constants used in the Herschel Bulky model from exp data (shear stress vs shear rate) to elaborate flow type of fluids ?

How to calculate various constants like consistency index and flow behavior index, used in the Herschel Bulky model from exp data (shear stress vs shear rate) in Origin or excel? If I need to...

22 August 2020 8,904 7 View

What are the basis for determining assessment factors during chemical risk analysis?

I have EC50 and LC50 values and wanted to calculate PEC for different species in water. I have currently considered acute risk with an assessment factor of 100 based on a previous similar...

12 July 2020 4,562 2 View

Please suggest for the critical parameters that are to be evaluated during simulation of a solar cell in COMSOL multiphysics tool?

Presently I am implementing solar cell in COMSOL multiphysics 3D time-varying structure. Kindly suggest me for the basic parameters that can be evaluated from a simulation model for concluding the...

10 March 2020 830 4 View

Need a brief idea about efficiency increasing in solar cell with geometric parameters?

I need to know about what parameters can be taken for efficiency calculation in the solar cell by COMSOL simulation

05 March 2020 1,330 7 View

Storing tissue in Agarose for 24 hrs ?

Dear All, I want to perform tissue section on different kinds of tissues like brain, muscle tissue,etc. I would like to store my fresh tissue in agarose for 24 hrs/over night before sectioning...

16 February 2020 878 2 View

How to differentiate T. elliptica, T. tomentosa, T. crenulata and T. alata? Different or synonymous?

Going through every literature and floral publication, they have their own theories, some say different species some consider synonymous to each other. There is a need of Key to Terminalia species...

08 September 2019 3,029 3 View

No title

please let me know about the journal

10 October 2018 4,004 3 View

How to know whether dopant ions are occupying interstitial sites in the lattice OR, replacing the host cations from their lattice positions?

I have prepared Y2O3 nanoparticles doped with 3% Ag & 3% Tb (at. wt. %). Now, how can I know the locations of Ag+ & Y3+ ions in the doped Y2O3 crystal? Also how we can keep the charge balance in...

15 September 2018 5,875 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View