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Questions related from Saurav Saha
Hello I am using OxPhos Rodent WB Antibody for mitochondrial protein expression study from muscle sample. But I faced difficulty for getting 5 different mitochondrial complexes after using this...
27 March 2023 7,747 2 View
We are trying to isolate nuclei from mouse skeletal muscle. After the isolation process, the clumping of nuclei was still present in the slide. This type of clumping nuclei is not acceptable for...
08 November 2022 9,358 0 View
I want to localize the pinealocytes, using immunohistochemistry. Generally, the pineal organ of the catfish is very tiny and thread like appearance. Sectioning of the pineal organ is too...
21 September 2018 7,982 9 View
Is there any type of circadian clock rhythmic expression happens due to different colors of light? Or different intensity of the light can affects? Kindly give me any suggestion. Thanks
02 September 2016 5,414 2 View
I have isolated RNA from fish retina using Tri reagent. After adding isopropanol, a black coloured RNA pellet has settled down. What is the possible reason behind this unusual black coloured...
08 June 2016 9,826 6 View
What is the difference between gene silencing and gene knockout? Kindly anyone explain me.
04 March 2016 324 7 View
I know this a one type of chronobiological disorder. Can anyone tell me what is the actual reason of this type of disorder? Because I feel myself this type of problem last few months. Any...
30 January 2016 5,711 2 View
Due to different photoperiod such as under 12L-12D, short day, long day, LL and DD condition the fishes were showed different clock gene expression on particular fish pineal. I am working on...
29 January 2016 6,011 3 View
I wanted to know any affect of continuous light (LL) and continuous darkness (DD) on the circadian rhythm of clock genes expression on fish pineal at 24 hours time period, mainly clock and bmal...
11 January 2016 2,148 3 View
Clock genes are controlled by the temperature and photoperiod. But I wanted to know different temperatures like 150 c,250 c,350 c so on.How to regulate the expression of clock genes of different...
16 December 2015 5,494 5 View
How to increase the stability of the competence cells? We are using Rubidium chloride, transformation for competent cells is arguably the compound's most abundant use. I would like to know...
01 January 1970 6,055 6 View
We are trying to isolate nuclei from mouse skeletal muscle but at the end of the process, the clumping of nuclei was still present in the slide. We were not getting good-quality of nuclei for...
01 January 1970 7,694 1 View
