It depends on the specific goals and needs of the situation. However, in general, identifying a pure compound is crucial before enhancing its production. This is because without knowing the exact chemical composition and structure of the bioactive compound, it would be difficult to optimize production processes effectively. Identifying a pure compound allows for better understanding of its properties, potential applications, and potential side effects. Once the compound is identified, efforts can then be focused on enhancing its production through various methods such as genetic engineering, fermentation optimization, or process scale-up.
The answer to this question depends on the specific goals and context of your research. If your aim is to increase the amount of bioactive compounds produced, this could lead to higher yields and potential therapeutic uses. On the other hand, focusing on isolating pure compounds is valuable for gaining a detailed understanding of their properties. Ultimately, the choice between these two objectives depends on the particular aims of your study.