I am working on plant extractd and I want to know why I should you mice instead of Wistar rats vice versa..
We are carrying out a research on software sizing and we observed there is need to track changes in requirement if adequate result is to be gotten. So, we opted for traceability matrix. Are we in...
25 February 2023 1,900 1 View
We are applying k-means clustering algorithm on an unlabeled data. Our aim is to at the end, pool a result that shows two possibility. Is it necessary then to carry out k-nn classification after...
25 February 2023 3,944 5 View
hello, I'm a presently running my master degree programme in cybersecurity and i am looking at Digital Forensics and Information security management. I really need good topics and materials on...
30 January 2023 3,972 7 View
i am working on noise level prediction, all needed data has been collected. the permissible exposure limit and recommended exposure limit noise level has also been determined. i want to predict...
20 February 2022 260 4 View
I am working plant extracts
30 October 2021 6,176 6 View
I am think Multiplex is better in terms of optimization and cost but might not be better in terms of ease of doing/handling.
30 October 2021 6,896 4 View
I need a general guide (simplified) on the usage of Wellcat software. Also, any idea/publication on how to further interpret core porosity and permeability data (apart from the routine...
10 July 2019 7,611 0 View
As I have seen publications with 2 and 4 levels RSM-CCD DOE. I will like to know if such designs are valid. Response Surface Methodology (RSM), Central Composite Design (CCD), Design of Experiment...
19 June 2019 4,514 12 View
I've been learning GIS. I use arcGIS for all my practical exercises. I want to have a profile for smallholder maize farmers within a selected area and also to map their land area using GIS. How...
26 March 2018 277 6 View
Any researcher willing to collaborate with our research group on different aspects of edible oils and fats analyses
11 February 2018 1,813 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View