Hi
Does anyone know which E. coli strain has higher outer membrane permeability, between MG1655 and BW25113?
Thanks
Hello Everyone I am performing a transfection of RPE-1 cell with some plasmid that contains blasticidin resistance. Anyone knows the concentration or range that I should use? Thanks!
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I read two references regarding this question. Reference 1 says that FM4-64 majorly stains inner membrane. But reference 2 says FM4-64 stains outer membrane. Anyone knows how does FM4-64 exactly...
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Hi. I have some questions about the movement of bacteria inside macrophage. 1) Anyone knows that whether bacteria will move around inside macrophage? 2)Also, once bacteria get uptake by...
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Hi. I'd like to insert two plasmids to E. coli strain, but 1. I wonder whether they are compatible. One plasmid is pTrc99A of which the origin is pBR322. And the other one is pASK-IBA3 plus, of...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
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I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
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Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View